Fatty acids by high-performance liquid chromatography and evaporative light-scattering detector

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• 作者：Elisabetta Bravi ; Giuseppe Perretti and Luigi Montanari
• 关键词：HPLC ; ELSD ; Fatty acids
• 刊名：Journal of Chromatography A
• 出版年：2006
• 出版时间：17 November 2006
• 年：2006
• 卷：1134
• 期：1-2
• 页码：210-214
• 全文大小：128 K

文摘

A high-performance liquid chromatographic (HPLC) separation method with an evaporative light-scattering detector (ELSD) has been developed for the separation and quantitative analysis of fatty acid methyl esters (FAME) in three different oils. Reverse-phased C₁₈ HPLC separation of 13 FAME is achieved using a methanol/water eluent mixture. The retention times (RT) reflect the elution behavior of these compounds on C₁₈ reversed-phase HPLC. The proposed method is tested on: soybean oil (Glycine max L.) as reference sample, rice bran oil (Oryza sativa L.), pumpkin seed oil (Cucurbita pepo L.) and algal oil (Arthrospira platensis Nordst.).