Calcitonin gene-related peptide immunoreactive sensory neurons in the vagal and glossopharyngeal ganglia innervating the larynx of the rat
详细信息 查看全文
- 作者：Tetsu Hayakawa ; tetsu@hyo-med.ac.jp" class="auth_mail ; Sachi Kuwahara-Otani ; Seishi Maeda ; Koichi Tanaka ; Makoto Seki
- 关键词：CGRP ; calcitonin gene-related peptide ; CTb ; cholera toxin subunit B ; DAB ; 3 ; 3&prime ; -diaminobenzidine ; FG ; Fluorogold ; HRP ; horseradish peroxidase ; IJV ; internal jugular vein ; ir ; immunoreactive ; JF ; jugular foramen ; ND ; nodose ganglion ; NTS ; nucleus tractus solitarii ; n10 ; vagus nerve ; n9 ; glossopharyngeal nerve ; PACAP ; pituitary adenylate cyclase-activating polypeptide ; PB ; phosphate buffer (pH 7.4) ; RLN ; recurrent laryngeal nerve ; SGPJG ; superior glossopharyngeal&ndash ; jugular ganglion comple
- 刊名：Journal of Chemical Neuroanatomy
- 出版年：January, 2014
- 年：2014
- 卷：55
- 期：Complete
- 页码：18-23
- 全文大小：1679 K
文摘
We have examined whether calcitonin gene-related peptide-immunoreactive (CGRP-ir) neurons in the vagal and glossopharyngeal ganglia innervate the larynx. Many CGRP-ir neurons were located mostly in the superior glossopharyngeal-jugular ganglion complex that was fused the superior glossopharyngeal ganglion and the jugular ganglion in the cranial cavity. When Fluorogold was applied to the cut end of the superior laryngeal nerve (SLN) or the recurrent laryngeal nerve (RLN), many Fluorogold-labeled neurons were found in the superior glossopharyngeal-jugular ganglion complex and the nodose ganglion. Double-labeling for CGRP and Fluorogold showed that about 80% of Fluorogold-labeled neurons in the superior glossopharyngeal-jugular ganglion complex expressed CGRP-like immunoreactivity in the case of application to the SLN, and about 50% of Fluorogold-labeled neurons expressed CGRP-like immunoreactivity in the case of the RLN. Only a few double-labeled neurons were found in the nodose ganglion. The number of the Fluorogold-labeled neurons and double-labeled neurons in the superior glossopharyngeal-jugular ganglion complex in the case of the SLN was larger than that in the case of the RLN. These results indicate that sensory information from the larynx might be conveyed by many CGRP-ir neurons located in the superior glossopharyngeal-jugular ganglion complex by way of the SLN and the RLN.