Puumala virus RNA in cerebrospinal fluid in a patient with uncomplicated nephropathia epidemica

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• 作者：Saara-Mari Mä ; hö ; nen ; Tarja Sironen ; Olli Vapalahti ; Eija Pä ; ä ; kkö ; Nina Hautala ; Jorma Ilonen ; Virpi Glumoff ; Olli Vainio ; Heikki Kauma ; Antti Vaheri ; Alex ; er Plyusnin ; Timo Hau
• 关键词：Puumala virus ; Meningitis ; Cerebrospinal fluid
• 刊名：Journal of Clinical Virology
• 出版年：2007
• 出版时间：November 2007
• 年：2007
• 卷：40
• 期：3
• 页码：248-251
• 全文大小：195 K

文摘

Nasopharyngeal secretions aspirated from infants with bronchiolitis (NPA) are a valuable resource for the study of virus dynamics and local inflammatory responses, however samples are small and difficult to manipulate.

Objectives

To improve yield of NPA from infants. To establish if removal of the cellular component of NPA affects quantification of human metapneumovirus (hMPV) or human respiratory syncytial virus (hRSV) genome.

Study design

Weight of NPA collected into traps from 30 infants was compared with that collected in trap plus catheter and washed through with saline from another 30 infants. hMPV (n = 33) and hRSV (n = 30) genome was measured by reverse-transcribed real-time polymerase chain reaction (RT-RT-PCR) in paired whole and cell-free NPA collected by the improved method.

Results

The improved method of NPA collection gave near two-fold greater weight (p = 0.002) of NPA (mean = 0.52 g (S.D. = 0.30 g)) than the traditional method (0.32 g (S.D. 0.19)). There was strong agreement and no significant difference between viral load measured in whole and cell-free fractions of NPA for both viruses (samples (n), correlation coefficient (cc) and significance (p)); hMPV (n = 33, cc = 0.938, p < 0.001) and hRSV (n = 30, cc = 0.977 and p < 0.001).

Conclusions

The majority of hRSV and hMPV in nasal secretions is not associated with cells. Removal of the cellular component of NPA does not interfere with quantification of hRSV and hMPV.