Separation and identification of phosphatidylserine molecular species using reversed-phase high-performance liquid chromatography with evaporative light scattering and mass spectrometric detection

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• 作者：Larsen ; Å ; smund ; Mokastet ; Eva ; Lundanes ; Elsa ; Hvattum ; Erlend
• 关键词：Evaporative light scattering detection ; Phosphatidylserines
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2002
• 出版时间：July 5, 2002
• 年：2002
• 卷：774
• 期：1
• 页码：115-120
• 全文大小：158 K

文摘

A reversed-phase HPLC method compatible with evaporative light scattering (ELS) and electrospray mass spectrometric (ES-MS) detection was developed for separation of phosphatidylserine (PS) molecular species. The method was optimised for separation of three disaturated synthetic species: dipalmitoyl glycerophosphoserine, palmitoyl-stearoyl glycerophosphoserine and distearoyl glycerophosphoserine using isocratic elution with a mixture of 2-propanol, tetrahydrofuran and ammonium formate. Baseline separation was obtained on three different columns: one polystyrene/divinylbenzene (PS/DVB) column and two silica based C₁₈ and C₃₀ columns. The best chromatographic resolution was achieved with the C₃₀ column. The limit of detection for DPPS was 5 μg/ml (S/N