Antiplasmodial, anti-complement and anti-inflammatory in vitro effects of Biophytum umbraculum Welw. traditionally used against cerebral malaria in Mali

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• 作者：Ingvild Austarheim^a ; ^{ingvilau@farmasi.uio.no" class="auth_mail" title="E-mail the corresponding author} ; Anh Thu Pham^a ; ^{anhthu.pham@abbvie.com" class="auth_mail" title="E-mail the corresponding author} ; Celine Nguyen^a ; ^{nguyen.celine@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Yuan-Feng Zou^a ; ¹ ; ^{yuanfengzou860315@163.com" class="auth_mail" title="E-mail the corresponding author} ; Drissa Diallo^b ; ^{dri.diallo@yahoo.fr" class="auth_mail" title="E-mail the corresponding author} ; Karl Egil Malterud^a ; ^{k.e.malterud@farmasi.uio.no" class="auth_mail" title="E-mail the corresponding author} ; Helle Wangensteen^a ; ^{helle.wangensteen@farmasi.uio.no" class="auth_mail" title="E-mail the corresponding author}
• 关键词：CM ; cerebral malaria ; EtOAc ; ethyl acetate ; MeOH ; methanol ; BuOH ; butanol ; DCM ; dichloromethane ; SI ; Selectivity Index ; RAW 264.7 ; murine macrophage cell line ; NO ; Nitric Oxide
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：22 August 2016
• 年：2016
• 卷：190
• 期：Complete
• 页码：159-164
• 全文大小：397 K

文摘

Biophytum umbraculum Welw. (Oxalidaceae) is a highly valued African medicinal plant used for treatment of cerebral malaria, a critical complication of falciparum malaria.

Aim of the study

To provide additional information about traditional use of B. umbraculum and to test plant extracts and isolated compounds for in vitro activities related to cerebral malaria.

Materials and methods

The traditional practitioners were questioned about indication, mode of processing/application, dosage and local name of B. umbraculum. Organic extracts and some main constituents of the plant were investigated for anti-malaria, anti-complement activity and inhibition of NO secretion in a RAW 264.7 cell line.

Results

Treatment of cerebral malaria was the main use of B. umbraculum (fidelity level 56%). The ethyl acetate extract showed anti-complement activity (ICH₅₀ 5.7±1.6 μg/ml), inhibition of macrophage activation (IC₅₀ 16.4±1.3 μg/ml) and in vitro antiplasmodial activity (IC₅₀ K1 5.6±0.13 μg/ml, IC₅₀ NF54 6.7±0.03 μg/ml). The main constituents (flavone C-glycosides) did not contribute to the activity of the extract.

Conclusion

Inhibition of complement activation and anti-inflammatory activity of B. umbraculum observed in this study might be possible targets for adjunctive therapy in cerebral malaria together with its antiplasmodial activity. However, clinical trials are necessary to evaluate the activity due to the complex pathogenesis of cerebral malaria.