“Light-up” Sensing of human 8-oxoguanine DNA glycosylase activity by target-induced autocatalytic DNAzyme-generated rolling circle amplification
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- 作者：Xiang-Juan Kong ; Shuang Wu ; Yao Cen ; Ru-Qin Yu ; Xia Chu ; xiachu@hnu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：hOGG1 ; Target-induced dual signal amplification strategy ; Autocatalytic DNAzyme ; Rolling circle amplification
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：79
- 期：Complete
- 页码：679-684
- 全文大小：1280 K
文摘
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A novel "light-up" sensor for human 8-oxoguanine DNA glycosylase activity assay was developed.
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The work mainly relies on target-induced formation of 5′ phosphorylated probe and autocatalytic DNAzyme-generated rolling circle amplification strategy.
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The assay exhibited a low detection limit of 0.001 U/mL for hOGG1.
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The strategy showed excellent specificity over other nonspecific enzymes and good analytical performance in complex biological media.