DNA methylation within melatonin receptor 1A (MTNR1A) mediates paternally transmitted genetic variant effect on asthma plus rhinitis

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• 作者：Chloé ; Sarnowski ; PhD^a ; ^b ; Catherine Laprise ; PhD^c ; Giovanni Malerba ; PhD^d ; Miriam F. Moffatt ; DPhil^e ; Marie-Hé ; l&egrave ; ne Dizier ; PhD^a ; ^b ; André ; anne Morin ; MSc^c ; ^h ; Quentin B. Vincent ; MD ; PhD^f ; ^g ; Klaus Rohde ; PhDⁱ ; Jorge Esparza-Gordillo ; PhDⁱ ; ^j ; Patricia Margaritte-Jeannin ; PhD^a ; ^b ; Liming Liang ; PhD^k ; Young-Ae Lee ; MDⁱ ; Jean Bousquet ; MD^l ; Valé ; rie Siroux ; PhD^m ; ⁿ ; ^o ; Pier Franco Pignatti ; MD^d ; William O. Cookson ; MD ; DPhil^e ; Mark Lathrop ; PhD^h ; Tomi Pastinen ; MD^h ; Florence Demenais ; MD^a ; ^b ; Emmanuelle Bouzigon ; MD ; PhD^a ; ^b ; ^{emmanuelle.bouzigon@inserm.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Linkage analysis ; positional cloning ; asthma ; allergic rhinitis ; parent-of-origin effect ; DNA methylation ; genetic association study
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：138
• 期：3
• 页码：748-753
• 全文大小：567 K

文摘

Asthma and allergic rhinitis (AR) are common allergic comorbidities with a strong genetic component in which epigenetic mechanisms might be involved.

Objective

We aimed to identify novel risk loci for asthma and AR while accounting for parent-of-origin effect.

Methods

We performed a series of genetic analyses, taking into account the parent-of-origin effect in families ascertained through asthma: (1) genome-wide linkage scan of asthma and AR in 615 European families, (2) association analysis with 1233 single nucleotide polymorphisms (SNPs) covering the significant linkage region in 162 French Epidemiological Study on the Genetics and Environment of Asthma families with replication in 154 Canadian Saguenay-Lac-Saint-Jean asthma study families, and (3) association analysis of disease and significant SNPs with DNA methylation (DNAm) at CpG sites in 40 Saguenay-Lac-Saint-Jean asthma study families.

Results

We detected a significant paternal linkage of the 4q35 region to asthma and allergic rhinitis comorbidity (AAR; P = 7.2 × 10⁻⁵). Association analysis in this region showed strong evidence for the effect of the paternally inherited G allele of rs10009104 on AAR (P = 1.1 × 10⁻⁵, reaching the multiple-testing corrected threshold). This paternally inherited allele was also significantly associated with DNAm levels at the cg02303933 site (P = 1.7 × 10⁻⁴). Differential DNAm at this site was found to mediate the identified SNP-AAR association.

Conclusion

By integrating genetic and epigenetic data, we identified that a differentially methylated CpG site within the melatonin receptor 1A (MTNR1A) gene mediates the effect of a paternally transmitted genetic variant on the comorbidity of asthma and AR. This study provides a novel insight into the role of epigenetic mechanisms in patients with allergic respiratory diseases.