- 作者：Bojana Mirković ; PhD<sup>asup><sup> ; sup><sup>&lowast ; sup> ; Gillian M. Lavelle ; BSc<sup>asup><sup> ; sup><sup>&lowast ; sup> ; Ahmed Abdul Azim ; MB ; BCh ; BAO<sup>asup> ; Kristine Helma ; MD<sup>asup> ; Fatma S. Gargoum ; MD<sup>asup> ; Kevin Molloy ; MB ; BCh ; BAO<sup>asup> ; Yael Gernez ; MD ; PhD<sup>bsup> ; Katie Dunne ; PhD<sup>csup> ; Julie Renwick ; PhD<sup>csup> ; Philip Murphy ; MD<sup>csup> ; Richard B. Moss ; MD<sup>dsup> ; Catherine M. Greene ; PhD<sup>asup> ; Cedric Gunaratnam ; MB<sup>asup> ; Sanjay H. Chotirmall ; MD ; PhD<sup>esup><sup> ; sup><sup>schotirmall@ntu.edu.sg" class="auth_mail" title="E-mail the corresponding authorsup> ; Noel G. McElvaney ; MD<sup>asup>
- 关键词：Allergic bronchopulmonary aspergillosis ; Aspergillus fumigatus ; basophil activation test ; body mass index ; CD203c ; cystic fibrosis ; flow cytometry ; FEV1 ; itraconazole ; sensitization
- 刊名：Journal of Allergy and Clinical Immunology
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：137
- 期：2
- 页码：436-443.e9
- 全文大小：1479 K
sSec_2">Objective
spara0015">We sought to identify A fumigatus sensitization in patients with CF by using the basophil activation test (BAT).
sSec_3">Methods
spara0020">Patients with CF attending Beaumont Hospital were screened for study inclusion. BAT was used to identify A fumigatus sensitization. Serologic (total and A fumigatus–specific IgE), pulmonary function, and body mass index measurements were performed.
sSec_4">Results
spara0025">The BAT discriminates A fumigatus–sensitized from nonsensitized patients with CF. Persistent isolation of A fumigatus in sputum is a significant risk factor for A fumigatus sensitization. Levels of the A fumigatus–stimulated basophil activation marker CD203c inversely correlated with pulmonary function and body mass index in A fumigatus–sensitized but not nonsensitized patients with CF. Total and A fumigatus–specific IgE, but not IgG, levels are increased in A fumigatus–sensitized patients with CF and ABPA when compared with those in A fumigatus–sensitized and nonsensitized patients with CF without ABPA. Itraconazole treatment did not affect A fumigatus sensitization.
sSec_5">Conclusion
spara0030">Combining the BAT with routine serologic testing allows classification of patients with CF into 3 groups: nonsensitized, A fumigatus–sensitized, and ABPA. Accurate and prompt identification of A fumigatus–associated clinical status might allow early and targeted therapeutic intervention, potentially improving clinical outcomes.