Development of a monolith based immobilized lipase micro-reactor for biocatalytic reactions in a biphasic mobile system

详细信息    查看全文

• 作者：Ping He ; Gillian Greenway ; Stephen J. Haswell
• 关键词：Micro-reactor ; Immobilized lipase ; Monolith ; Biphasic system ; Hydrolysis reaction ; Enzyme kinetics
• 刊名：Process Biochemistry
• 出版年：2010
• 出版时间：April 2010
• 年：2010
• 卷：45
• 期：4
• 页码：593-597
• 全文大小：279 K

文摘

This paper reports a simple method for producing macroporous silica-monoliths with controllable porosity that can be used for the immobilization of lipases to generate an active and stable micro-reactor for biocatalysis. A range of commercially available lipases has been examined using the hydrolysis reactions of 4-nitrophenyl butyrate in water–decane media. The kinetic studies performed have identified that a similar value for k_cat is obtained for the immobilized Candida antarctica lipase A (0.13 min⁻¹) and the free lipase in solution (0.12 min⁻¹) whilst the immobilized apparent Michaelis constant K_m (3.1 mM) is 12 times lower than the free lipase in solution (38 mM). A 96 % conversion was obtained for the immobilized C. antarctica lipase A compared to only 23 % conversion for the free lipase. The significant higher conversions obtained with the immobilized lipases were mainly attributed to the formation of a favourable biphasic system in the continuous flowing micro-reactor system, where a significant increase in the interfacial activation occurred. The immobilized C. antarctica lipase A on the monolith also exhibited improved stability, showing 64 % conversion at 80 °C and 70 % conversion after continuous running for 480 h, compared to 40 and 20 % conversions under the same temperature and reaction time for the free lipase.