Stability and disassembly properties of human naïve Hsp60 and bacterial GroEL chaperonins
详细信息 查看全文
- 作者：Caterina Riccia ; Maria Grazia Ortorea ; m.g.ortore@univpm.it" class="auth_mail" title="E-mail the corresponding author ; Silvia Vilasib ; Rita Carrottab ; Maria Rosalia Mangioneb ; Donatella Buloneb ; Fabio Librizzib ; Francesco Spinozzia ; Giosalba Burgioc ; Heinz Amenitschd ; Pier Luigi San Biagiob
- 关键词：Hsp60 ; GroEL ; SAXS ; CD ; Guanidinium chloride ; Denaturation
- 刊名：Biophysical Chemistry
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：208
- 期：Complete
- 页码：68-75
- 全文大小：1049 K
文摘
- •
Human Hsp60 chaperonin and its bacterial homolog GroEL were studied in solution.
- •
Native GroEL is tetradecameric, while Hsp60 presents tetradecamers and heptamers.
- •
Adding guanidine hydrochloride, both dissociation and unfolding processes occur.
- •
Hsp60 is less stable in respect to GroEL.
- •
Differently from Hsp60, GroEL disassembly has no heptameric intermediate.