Comprehensive analysis of glucose and xylose metabolism in Escherichia coli under aerobic and anaerobic conditions by ¹³C metabolic flux analysis

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• 作者：Jacqueline E. Gonzalez ; Christopher P. Long ; Maciek R. Antoniewicz ; ^{mranton@udel.edu}
• 关键词：Parallel labeling experiments ; Metabolism ; Model validation ; Flux estimation ; Co-factor balances
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：9-18
• 全文大小：1729 K
• 卷排序：39

文摘

Parallel labeling experiments were applied to measure precise metabolic fluxes in E. coli. Glucose and xylose metabolism was quantified during aerobic and anaerobic growth. During anaerobic growth, TCA cycle is bifurcated and oxidative PPP flux is reduced. During anaerobic growth, biomass turnover produces CO2 for anaplerosis. β-oxidation is required for anaerobic growth on xylose.