Role of the inhibition of oxidative stress and inflammatory mediators in the neuroprotective effects of hydroxytyrosol in rat brain slices subjected to hypoxia reoxygenation

详细信息    查看全文

• 作者：Susana Cabrerizo ; Jos茅 Pedro De La Cruz ; Juan Antonio L贸pez-Villodres ; Javier Mu帽oz-Mar铆n ; Ana Guerrero ; Jos茅 Julio Reyes ; Mar铆a Teresa Labajos ; Jos茅 Antonio Gonz谩lez-Correa
• 关键词：GSH ; reduced glutathione ; GSHpx ; glutathione peroxidase ; GSHtf ; glutathione transferase ; GSSG ; oxidized glutathione ; HT ; hydroxytyrosol ; IL-1尾 ; interleukin 1尾 ; LDH ; lactate dehydrogenase ; MDA ; malondialdehyde ; NO-2 + NO-3 ; nitrites + nitrates ; PGE2 ; prostaglandin E2 ; TBARS ; thiobarbituric acid reactive substances
• 刊名：Journal of Nutritional Biochemistry
• 出版年：December, 2013
• 年：2013
• 卷：24
• 期：12
• 页码：2152-2157
• 全文大小：540 K

文摘

The aim of this study was to analyze the mechanism of the neuroprotective effect of hydroxytyrosol (HT) in an experimental model of hypoxia-reoxygenation in rat brain slices. After reoxygenation the increase in lactate dehydrogenase efflux was inhibited by HT in a concentration-dependent manner and dose-dependent inhibition after oral administration to rats for 7 days (1, 5 and 10 mg/kg per day). Maximum inhibition was 57.4% in vitro and 38.7% ex vivo. Hydroxytyrosol reduced oxidative stress parameters: it inhibited lipid peroxidation and increased enzymatic activities related with the glutathione system both in vitro and after oral administration to rats. The increase in prostaglandin E₂ and interleukin 1尾 after reoxygenation were inhibited after incubation of brain slices with HT and after oral administration. The accumulation of nitric oxide in brain slices was reduced in a concentration-dependent manner. In conclusion, HT exerts a neuroprotective effect in a model of hypoxia-reoxygenation in rat brain slices, both in vitro and after 7 days of oral administration to rats. HT exerts an antioxidant activity and lowered some inflammatory markers in this model.