The effect of silicon on the uptake and translocation of arsenic in tomato (Solanum lycopersicum L.)

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• 作者：M. Marmiroli ; V. Pigoni ; M.L. Savo-Sardaro ; N. Marmiroli
• 关键词：As III ; arsenite ; As V ; arsenate ; AFLP ; amplified fragment length polymorphisms ; cv. ; cultivar ; DMA ; dimethylarsinic acid ; D.W. ; dry weight ; exp ; expressed sequence ; F.W. ; fresh weight ; GDA ; genetic data analysis ; GSH ; glutathione ; GSSH ; reduced glutathione ; GST ; glutathione S transferase ; HG-AAS ; atomic absorption spectroscopy with hydride generation ; HSP 90-1 ; heat shock Protein 90-1 gene ; LeGR ; lycopersicum esculetum glutathione reductase gene ; MMA ; monomethylarsonic acid ; MS ; Murashige and S
• 刊名：Environmental and Experimental Botany
• 出版年：March, 2014
• 年：2014
• 卷：99
• 期：Complete
• 页码：9-17
• 全文大小：2330 K

文摘

Measuring tomato seed germination on a medium containing either arsenite or arsenate showed that the presence of 0.5 mM NaH₂AsO₄路7H₂O reduced germination by between 20% and 40%, depending on cultivar. The inhibitory effect was mitigated by the addition of CaSiO₃. However, the presence of both forms of As had a drastic negative effect on seedling shoot elongation, which was not mitigated by the presence of CaSiO₃. In a subsequent soil-based pot trial, damage due to the presence of As was visible by 15 days after the initiation of the treatment, and the provision of CaSiO₃ was significantly ameliorative; again, the severity of the effects was cultivar-dependent. Analysis of the accumulation and distribution of As showed that some of the cultivars are As excluders, and others accumulators. As was taken up by the latter cultivars whether or not CaSiO₃ supplementation was provided. The extent of As entry into the fruit varied from cultivar to cultivar, but never rose above the safety threshold. A survey of stress response-associated genes showed that LeGR was strongly up-regulated by exposure to As.