The fabrication of a piezoelectric immunosensor based on DNA–antibody conjugate layer

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• 作者：Guangyu Shen^a ;   ; ^{sgyrab@yahoo.com.cn} ; Jilin Lu^a ; Chenbo Cai^b
• 关键词：DNA&ndash ; antibody conjugate ; Antibody immobilization ; Piezoelectric immunosensor
• 刊名：Analytical Biochemistry
• 出版年：2011
• 出版时间：15 November 2011
• 年：2011
• 卷：418
• 期：2
• 页码：167-171
• 全文大小：435 K
• ISSN：S0003269711004362

文摘

In this article, we report a method of antibody immobilization carried out by hybridizing DNA–antibody conjugates on a mixed self-assembled monolayer composed of DNA thiols and mercaptopropionic acid via sequence-specific hybridization. The proposed method was applied to fabricate an immunosensor for detecting human immunoglobulin G (IgG). Under the optimized experimental conditions, a wide linear range from 50.0 to 500 μg/ml was reached with a detection limit of 30.13 μg/ml. The developed immunosensor possesses advantages such as simple fabrication, wide linear range, easy regeneration, and excellent reproducibility.