Inhibitory kinetics of chlorocinnamic acids on mushroom tyrosinase
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- 作者：Yong-Hua Hu ; Xuan Liu ; Yu-Long Jia ; Yun-Ji Guo ; Qin Wang ; Qing-Xi Chen
- 关键词：Chlorcinnamic acid ; Synthesis ; Mushroom tyrosinase ; Inhibition ; Kinetics
- 刊名：Journal of Bioscience and Bioengineering
- 出版年：February, 2014
- 年：2014
- 卷：117
- 期：2
- 页码：142-146
- 全文大小：857 K
文摘
Tyrosinase (EC 1.14.18.1) is the key enzyme of most food enzymatic oxidation. Tyrosinase inhibitors are important in food industry. In the present paper, 2-chlorcinnamic acid and 2,4-dichlorocinnamic acid were synthesized and the inhibitory kinetics on mushroom tyrosinase were investigated. The results showed that both compounds synthesized could inhibit tyrosinase activity. For monophenolase activity, both chlorocinnamic acids could extended the lag time and decrease the steady-state activities, 2-chlorcinnamic acid extended the lag time just by 5%, and 2,4-dichlorcinnamic acid extended the lag time more than by 30.4%. For diphenolase activity, the IC50 values of 2-chlorcinnamic acid and 2,4-dichlorocinnamic acid were determined to be 0.765聽mM and 0.295聽mM, respectively. The inhibition kinetics showed that 2-chlorcinnamic acid and 2,4-dichlorocinnamic acid displayed a reversible and uncompetitive mechanism. The inhibition constants were determined to be 0.348聽mM and 0.159聽mM, respectively. The research may supply the basis for designing new tyrosinase inhibitors.