Green tea polyphenols protect against okadaic acid-induced acute learning and memory impairments in rats

详细信息    查看全文

• 作者：Hongyu Li ; Xiukui Wu ; Qiong Wu ; Dezheng Gong ; Meijun Shi ; Lili Guan ; Jun Zhang ; Jing Liu ; Bo Yuan ; Guozhu Han ; Yuan Zou
• 关键词：Green tea polyphenols ; Tau protein ; Hyperphosphorylation ; Okadaic acid ; Neuroprotection
• 刊名：Nutrition
• 出版年：March, 2014
• 年：2014
• 卷：30
• 期：3
• 页码：337-342
• 全文大小：996 K

文摘

Objective

Green tea polyphenols (GTPs) are now being considered possible protective agents in neurodegenerative diseases such as Alzheimer's disease (AD). Previous studies suggested that GTPs could inhibit amyloid fibril formation and protect neurons from toxicity induced by 尾-amyloid. However, whether GTPs can ameliorate learning and memory impairments and also reduce tau hyperphosphorylation induced by okadaic acid (OA) in rats remains unclear. The aim of this study was to determine if GTPs have neuroprotection against OA-induced neurotoxicity.

Methods

In this work, rats were pretreated with GTPs by intragastric administration for 4 wk. Then OA was microinjected into the right dorsal hippocampus. Morris water maze tests were used to test the ethologic changes in all groups, and tau protein hyperphosphorylation was detected both in聽vivo and in聽vitro.

Results

The ethologic test indicated that the staying time and swimming distance in the target quadrant were significantly decreased after OA treatment, whereas rats pretreated with GTPs stayed longer in the target quadrant. Methyl thiazolyl tetrazolium assay and lactate dehydrogenase leakage showed that GTPs greatly ameliorated primary hippocampal neurons damage induced by OA. Furthermore, reduced hyperphosphorylated tau protein was detected with GTPs pretreatment.

Conclusion

Taken together, our results suggest that GTPs have neuroprotection against OA-induced neurotoxicity.