ITF2 Prevents Activation of the β-Catenin-TCF4 Complex in Colon Cancer Cells and Levels Decrease With Tumor Progression

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• 作者：Hyun&ndash ; Woo Shin¹ ; ² ; ³ ; Hyunsung Choi¹ ; Daeho So¹ ; ⁴ ; Young&ndash ; Im Kim¹ ; ⁴ ; Kumsun Cho⁴ ; Hee&ndash ; Joon Chung⁵ ; Kyoung&ndash ; Hwa Lee² ; ⁶ ; Yang&ndash ; Sook Chun² ; ⁴ ; ⁶ ; Chung&ndash ; Hyun Cho¹ ; ² ; ³ ; Gyeong Hoon Kang⁷ ; Woo Ho Kim⁷ ; Jong&ndash ; Wan Park¹ ; ² ; ³ ; ⁴ ; ^{parkjw@snu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Tumor Suppressor ; Wnt Signaling ; Signal Transduction ; Mouse Model
• 刊名：Gastroenterology
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：147
• 期：2
• 页码：430-442.e8
• 全文大小：5712 K

文摘

Immunoglobulin transcription factor 2 (ITF2) was believed to promote neoplastic transformation via activation of β-catenin. However, ITF2 recently was reported to suppress colon carcinogenesis. We investigated the roles of ITF2 in colorectal cancer cell lines and tumor formation and growth in mice.

Methods

Levels of ITF2, β-catenin, and c-Myc were measured in 12 human colorectal tumor samples and by immunohistochemistry. ITF2 regulation of β-catenin and T-cell factor (TCF) were analyzed using luciferase reporter, reverse-transcription quantitative polymerase chain reaction, flow cytometry, and immunoblot analyses. Mice were given subcutaneous injections of human colorectal cancer cell lines that stably express ITF2, small hairpin RNAs to reduce levels of ITF2, or control plasmids; xenograft tumor growth was assessed. Human colorectal carcinoma tissue arrays were used to associate levels of ITF2 expression and clinical outcomes.

Results

Levels of β-catenin, cMyc, and ITF2 were increased in areas of human colon adenomas and carcinomas, compared with nontumor areas of the same tissues. ITF2 levels were reduced and cMyc levels were increased in areas of carcinoma, compared with adenoma. In human colorectal cancer cell lines, activation of the β-catenin–TCF4 complex and expression of its target genes were regulated negatively by ITF2. ITF2 inhibited formation of the β-catenin–TCF4 complex by competing with TCF4 for β-catenin binding. Stable transgenic expression of ITF2 in human colorectal cancer cell lines reduced their proliferation and tumorigenic potential in mice, whereas small hairpin RNA knockdown of ITF2 promoted growth of xenograft tumors in mice. In an analysis of colorectal tumor tissue arrays, loss of ITF2 from colorectal tumor tissues was associated with poor outcomes of patients. A gene set enrichment analysis supported the negative correlation between the level of ITF2 and activity of the β-catenin–TCF4 complex.

Conclusions

In human colorectal cancer cell lines and tissue samples, ITF2 appears to prevent activation of the β-catenin–TCF4 complex and transcription of its gene targets. Loss of ITF2 promotes the ability of colorectal cancer cells to form xenograft tumors, and is associated with tumor progression and shorter survival times of patients.