¹H NMR-based metabonomics study on the toxicity alleviation effect of other traditional Chinese medicines in Niuhuang Jiedu tablet to realgar (As₂S₂)

详细信息    查看全文

• 作者：Wenfeng Xu^a ; Haifeng Wang^a ; Gang Chen^a ; Wen Li^b ; Rongwu Xiang^c ; Yuehu Pei^a ; ^{peiyueh@vip.163.com}
• 关键词：Metabonomics ; Toxicity alleviation ; Niuhuang Jiedu Tablet ; NMR spectroscopy ; Reaglar (As2S2)
• 刊名：Journal of Ethnopharmacology
• 出版年：2013
• 出版时间：21 June, 2013
• 年：2013
• 卷：148
• 期：1
• 页码：88-98
• 全文大小：4329 K

文摘

Ethnopharmacological relevance

Niuhuang Jiedu Tablet (NJT) is an effective prescription of traditional Chinese medicine (TCM) used in treating acute tonsillitis, pharyngitis, periodontitis and mouth ulcer. NJT is prepared from Xionghuang (Realgar, As₂S₂), Rengong Niuhuang (Bovis Calculus Artificialis), Bingpian (Borneolum Synthcticum), Shigao (Gypsum Fibrosum), Dahuang (Rhei Radix et Rhizoma), Huangqin (Scutellariae Radix), Jiegeng (Platycodonis Radix) and Gancao (Glycyrrhizae Radix et Rhizoma). In the prescription, significant level of realgar (As₂S₂) as a potentially toxic element is contained.

Aim of the study

In this study, ¹H NMR-based metabonomics approach has been used to investigate the toxicity of realgar (As₂S₂) after being counterbalanced by other TCMs in NJT.

Materials and methods

Male Wistar rats were divided into five groups: control, group I (treated with Realgar), group II (treated with Realgar, Bovis Calculus Artificialis, Borneolum Synthcticum, Gypsum Fibrosum, Rhei Radix et Rhizoma, Scutellariae Radix, Platycodonis Radix and Glycyrrhizae Radix et Rhizoma), group III (treated with Realgar, Bovis Calculus Artificialis, Borneolum Synthcticum and Gypsum Fibrosum) and group IV (treated with Realgar, Rhei Radix et Rhizoma, Scutellariae Radix, Platycodonis Radix and Glycyrrhizae Radix et Rhizoma). Based on ¹H-NMR spectra of urine and serum from rats, PCA and PLS-DA were performed to identify different metabolic profiles. Liver and kidney histopathology examinations and serum clinical chemistry analysis were also performed.

Results

PLS-DA scores plots demonstrated that the cluster of group I was separated from that of control rats, while group II was located close to control rats, indicating that metabolic profiles of group II were restored toward those of control rats. The metabolic profiles of group III were similar to those of group I, while the metabolic profiles of group II were almost in line with those of group II. Statistics results were confirmed by the histopathological examination and biochemical assay.

Conclusion

Our results indicated that it was more secure and much less toxic for counterbalanced realgar (As₂S₂) in NJT. The effective material bases of toxicity alleviation to realgar (As₂S₂) were Dahuang (Rhei Radix et Rhizoma), Huangqin (Scutellariae Radix), Jiegeng (Platycodonis Radix) and Gancao (Glycyrrhizae Radix et Rhizoma), which regulated energy metabolism, choline metabolism, amino acid metabolism and gut flora disorder affected by realgar (As₂S₂) exposure.