Coenzyme Q₁₀ production by immobilized Sphingomonas sp. ZUTE03 via a conversion-extraction coupled process in a three-phase fluidized bed reactor

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• 作者：Lequan Qiu^a ; Hanbing Ding^a ; Weijian Wang^a ; Zhuoyi Kong^a ; Xuanzhen Li^a ; Yuping Shi^b ; ^{shiyuping007@hotmail.com} ; Weihong Zhong^a ; ^{whzhong@zjut.edu.cn}
• 关键词：CoQ10 ; Sphingomonas sp. ZUTE03 ; Three-phase fluidized bed reactor ; Conversion&ndash ; extract coupled process
• 刊名：Enzyme and Microbial Technology
• 出版年：2012
• 出版时间：10 February 2012
• 年：2012
• 卷：50
• 期：2
• 页码：137-142
• 全文大小：443 K

文摘

A three-phase fluidized bed reactor (TPFBR) was designed to evaluate the potential of CoQ₁₀ production by gel-entrapped Sphingomonas sp. ZUTE03 via a conversion-extract coupled process. In the reactor, the CoQ₁₀ yield reached 46.99 mg/L after 8 h of conversion; a high-level yield of about 45 mg/L was maintained even after 15 repetitions (8 h/batch). To fully utilize the residual precursor (para-hydroxybenzoic acid, PHB) in the aqueous phase, the organic phase was replaced with new solution containing 70 mg/L solanesol for each 8 h batch. The CoQ₁₀ yield of each batch was maintained at a level of about 43 mg/L until the PHB ran out. When solid solanesol was fed to the organic phase for every 8 h batch, CoQ₁₀ could accumulate and reach a yield of 171.52 mg/L. When solid solanesol and PHB were fed to the conversion system after every 8 h batch, the CoQ₁₀ yield reached 441.65 mg/L in the organic phase after 20 repetitions, suggesting that the conversion-extract coupled process could enhance CoQ₁₀ production in the TPFBR.