Regulation of acute graft-versus-host disease by human umbilical cord blood derived stromal cells in haploidentical stem cell transplantation in mice through very late activation antigen-4

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• 作者：Cheng Zhang¹ ; ^a ; ^{chzhang2003@tom.com} ; Xing-Hua Chen ; ^a ; ^{xhchen888@yahoo.com.cn} ; Xi Zhang¹ ; ^a ; ^{zhangxxi@sina.com.cn} ; Lei Gao¹ ; ^a ; ^{glei202009@163.com} ; Li Gao¹ ; ^a ; ^{gaoli339572@tom.com} ; Pei-Yan Kong¹ ; ^a ; ^{kpy102009@163.com} ; Xian-Gui Peng¹ ; ^a ; ^{pengxg2008@163.com} ; Ai-Hua Sun¹ ; ^a ; ^{sun1968aihua@163.com} ; Qing-Yu Wang¹ ; ^a ; ^{wagn39523qy@yahoo.cn}
• 关键词：Very late activation antigen-4 ; Human umbilical cord blood derived stromal cells ; Haploidentical transplantation ; Mice ; Graft-versus-host disease ; Human bone marrow stromal cells
• 刊名：Clinical Immunology
• 出版年：2011
• 出版时间：April 2011
• 年：2011
• 卷：139
• 期：1
• 页码：94-101
• 全文大小：709 K

文摘

Human umbilical cord blood derived stromal cells (hUCBDSCs), a novel resource isolated by our laboratory, have been shown to exert an immunologic regulation. Very late activation antigen-4 (VLA-4) has been associated with graft-versus-host disease (GVHD). This study aimed to investigate the possible mechanism by in vitro co-cultured splenocytes of donor mice with hUCBDSCs and in haploidentical stem cell transplantation in mice with acute GVHD. Both hUCBDSCs and human bone marrow stromal cells (hBMSCs) elicited decreased lymphocyte expression of VLA-4, but this decrease was stronger with hUCBDSCs than with hBMSCs (p < 0.05). Cotransplantation of bone marrow with hUCBDSCs significantly decreased the expression of VLA-4 compared with control mice (p < 0.05). A significant reduction of VLA-4 labeling in the target organs of GVHD was evident in haploidentical mice cotransplanted with hUCBDSCs. Our study shows that hUCBDSCs may protect mouse recipients of haploidentical stem cell transplantation from aGVHD via downregulating the expression of VLA-4.