Isolation and characterization of a novel antistress gene from Chlamydomonas sp. W80

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• 作者：Yoshito  ; Suda¹ ;   ; ^{yosi551@hotmail.com} ; Tomoaki  ; Yoshikawa² ;   ; ^{tomoaki@phs.osaka-u.ac.jp} ; Yuko  ; Okuda¹ ;   ; ^{okuda916yuuko@hotmail.co.jp} ; Mei  ; Tsunemoto¹ ;   ; ^{ph4046tm@phs.osaka-u.ac.jp} ; Satoshi  ; Tanaka³ ;   ; ^{tanaka.satoshi@a3.kepco.co.jp} ; Kazunori  ; Ikeda⁴ ;   ; ^{ikeda_kazunori@kanso.co.jp} ; Hitoshi  ; Miyasaka³ ;   ; ^{k448482@kepco.co.jp} ; Masanori  ; Watanabe⁵ ;   ; ^{watanabe@hkg.ac.jp} ; Ken  ; Sasaki⁵ ;   ; ^{sasaki@hkg.ac.jp} ; Kazuo  ; Harada¹ ;   ; ^{harada6@phs.osaka-u.ac.jp} ; Takeshi  ; Bamba¹ ;   ; ^{bamba@bio.eng.osaka-u.ac.jp} ; Kazumasa  ; Hirata¹ ;   ; ^{hirata@phs.osaka-u.ac.jp}
• 关键词：Chlamydomonas ; Anti-stress gene ; Green alga ; ATP synthase ; subunit CFo-II
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2009
• 出版时间：April 2009
• 年：2009
• 卷：107
• 期：4
• 页码：352-354
• 全文大小：424 K

文摘

Here we report the isolation and characterization of the Chlamydomonas sp. W80-derived CFo ATP synthase subunit II WCFII homologue gene. Intracellular ATP concentration and stress-tolerance of Escherichia coli were increased by transformation with WCFII. We propose that WCFII-mediated stress tolerance is due to enhanced accumulation of intracellular ATP.