Comparative higher-order structure analysis of antibody biosimilars using combined bottom-up and top-down hydrogen-deuterium exchange mass spectrometry
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- 作者：Jingxi Pana ; Suping Zhangb ; Christoph H. Borchersa ; c ; christoph@proteincentre.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Hydrogen/deuterium exchange (HDX) ; Top-down ; Bottom-up ; Antibody ; Biosimilar
- 刊名：Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：1864
- 期：12
- 页码：1801-1808
- 全文大小：1467 K
文摘
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HDX-MS provided comprehensive higher-order structural characterization of antibodies.
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Top-down and bottom-up data were complementary.
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Top-down ETD provided residue-level structural information of the light chain.
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Bottom-up provided better coverage of the heavy chain.