Methylanthraquinone from Hedyotis diffusa WILLD induces Ca²⁺-mediated apoptosis in human breast cancer cells

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• 作者：Zheng Liu ; Ming Liu ; Miao Liu ; Jianchun Li
• 关键词：Hedyotis diffusa WILLD ; Apoptosis ; Caspase ; Calpain ; Cytochrome c ; [Ca²⁺]_i
• 刊名：Toxicology in Vitro
• 出版年：2010
• 出版时间：February 2010
• 年：2010
• 卷：24
• 期：1
• 页码：142-147
• 全文大小：1763 K

文摘

Methylanthraquinone from Hedyotis diffusa WILLD exhibited potent anticancer activity in many kinds of cancer cells. However, the exact mechanism and signaling pathway involved in methylanthraquinone-induced apoptosis have not been fully elucidated. Therefore, we explored the mechanisms of methylanthraquinone-mediated apoptosis in MCF-7 human breast cancer cells. When MCF-7 cells were co-incubated with methylanthraquinone, the percentage of apoptotic cell and S phase of cell cycle was markedly increased. In addition, a rise in intracellular calcium levels, phosphorylation of JNK and activation of calpain were found in MCF-7 cells after exposure to methylanthraquinone. With the methylanthraquinone-mediated reduction of mitochondrial membrane potential, cytochrome c was released from mitochondria to cytosol. Moreover, methylanthraquinone strongly induced cleavage of caspase-4, caspase-9 and caspase-7 in MCF-7 cells. These results suggested that methylanthraquinone from Hedyotis diffusa WILLD induced MCF-7 cells apoptosis via Ca²⁺/calpain/caspase-4 pathway.