Comparison of four commercial DNA extraction kits for the recovery of Bacillus spp. spore DNA from spiked powder samples
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- 作者：Markos Mö ; lsä ; a ; markos.molsa@thl.fi" class="auth_mail" title="E-mail the corresponding author ; Laura Kalin-Mä ; nttä ; rib ; Elina Tonteria ; Heidi Hemmilä ; a ; Simo Nikkaria
- 关键词：DNA extraction ; Spore ; Powder ; Biothreat ; Bacillus spp. ; Bacillus thuringiensis ; Bacillus anthracis ; Spore inactivation
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：128
- 期：Complete
- 页码：69-73
- 全文大小：530 K
文摘
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Bacillus spp. spores may remain active over sample handling.
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Performance of manual DNA extraction kits were tested for sample inactivation.
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DNA samples were incubated on LB plates to evaluate the inactivation efficiency.
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Adding a DNA filtration step ensured the removal of viable bacteria and spores.
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Manual extraction kits are effective for fast DNA extraction in field conditions.