Evaluation and Minimization of Uncertainty in ITC Binding Measurements: Heat Error, Concentration Error, Saturation, and Stoichiometry
文摘
The uncertainty in injection heat for a VP-ITC microcalorimeter is roughly proportional to heat release Over a wide range of experimental conditions, fixing the stoichiometry, N, reduces uncertainty in calorimetry results, relative to letting N float Reducing the number of titration steps tends to degrade the precision of ITC results Binding thermodynamics can often be precisely fit from low C value plots, given that N is held fixed