Effect of prostaglandin E₂ and prostaglandin I₂ on PDGF-induced proliferation of LI90, a human hepatic stellate cell line

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• 作者：Alex Yui Hui ; Alfred Sze-Lok Cheng ; Henry Lik-Yuen Chan ; Minnie Yin-Yin Go ; Francis Ka-Leung Chan ; Ryuichiro Sakata ; Takato Ueno ; Michio Sata ; Joseph Jao-Yiu Sung
• 关键词：Methotrexate ; Heat shock protein ; Prostaglandin ; none ; color ; black"" href=""/science?_ob=MathURL&_method=retrieve&_udi=B6WPH-4DN94NJ-1&_mathId=mml126&_user=3986987&_cdi=6991&_rdoc=2&_acct=C000050221&_version=1&_
• 刊名：Prostaglandins, Leukotrienes and Essential Fatty Acids
• 出版年：2004
• 出版时间：November 2004
• 年：2004
• 卷：71
• 期：5
• 页码：329-333
• 全文大小：207 K

文摘

Hepatic stellate cells (HSC) are central to liver fibrosis. The eicosanoid pathway and cyclooxygenase-2 (COX-2) may be an important signaling mechanism in HSC. We investigated the role of COX-2, prostaglandin E₂ (PGE₂) and prostaglandin I₂ (PGI₂) in proliferation of LI90, an immortalized cell line of HSC. Our results showed that COX-2 was upregulated by platelet-derived growth factor (PDGF), a mitogen in HSC. COX-2 was responsible for the production of PGE₂ and PGI₂ in PDGF-stimulated LI90 cells. Furthermore, we demonstrated that COX-2 and PGE₂ mediated the proliferative response of LI90 to PDGF while synthetic analogue of PGI₂ exhibited anti-proliferative effect. Our findings suggest complex interactions of prostaglandins in liver fibrogenesis. In vivo studies using animal models are needed to elucidate the effect of COX-2 inhibition by non-steroidal anti-inflammatory drugs or COX-2 inhibitor in hepatic fibrosis.