Structure of the lipopolysaccharide core of Vibrio vulnificus
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- 作者：Evguenii Vinogradov ; Caroline Wilde ; Erin M. Anderson ; Alina Nakhamchik ; Joseph S. Lam ; Dean A. Rowe-Magnus
- 关键词：Vibrio vulnificus ; Lipopolysaccharide ; Core ; Structure
- 刊名：Carbohydrate Research
- 出版年：2009
- 出版时间：10 March 2009
- 年：2009
- 卷：344
- 期：4
- 页码：484-490
- 全文大小：682 K
文摘
The structure of the lipopolysaccharide core of Vibrio vulnificus type strain 27562 is presented. LPS hydrolysis gave two oligosaccharides, OS-1 and OS-2, as well as lipid A. NMR spectroscopic data corresponded to the presence of one Kdo residue, one β-glucopyranose, three heptoses, one glyceric acid, one acetate, three PEtN, and one 5,7-diacylamido-3,5,7,9-tetradeoxynonulosonic acid residue (pseudaminic acid, Pse) in OS1. OS2 differed form OS 1 by the absence of glyceric acid, acetate, and Pse residues. Lipid A was analyzed for fatty acid composition and the following fatty acids were found: C14:0, C12:0-3OH, C16:0, C16:1, C14:0-3OH, C18:0, C18:1 in a ratio of 1:3:3:1:2.5:0.6:0.8.