Quantitative determination of fatty acid compositions in micro-encapsulated fish-oil supplements using Fourier transform infrared (FTIR) spectroscopy
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- 作者：Jitraporn Vongsvivut ; Philip Heraud ; Wei Zhang ; Jaroslav A. Kralovec ; Don McNaughton ; Colin J. Barrow
- 关键词：Micro-encapsulated fish-oil supplement ; Polyunsaturated fatty acids (PUFAs) ; Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy ; Partial least square regression (PLSR) analysis
- 刊名：Food Chemistry
- 出版年：2012
- 出版时间：15 November, 2012
- 年：2012
- 卷：135
- 期：2
- 页码：603-609
- 全文大小：1357 K
文摘
The research describes a rapid method for the determination of fatty acid (FA) contents in a micro-encapsulated fish-oil (¦ÌEFO) supplement by using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic technique and partial least square regression (PLSR) analysis. Using the ATR-FTIR technique, the ¦ÌEFO powder samples can be directly analysed without any pre-treatment required, and our developed PLSR strategic approach based on the acquired spectral data led to production of a good linear calibration with R2 = 0.99. In addition, the subsequent predictions acquired from an independent validation set for the target FA compositions (i.e., total oil, total omega-3 fatty acids, EPA and DHA) were highly accurate when compared to the actual values obtained from standard GC-based technique, with plots between predicted versus actual values resulting in excellent linear fitting (R2 ? 0.96) in all cases. The study therefore demonstrated not only the substantial advantage of the ATR-FTIR technique in terms of rapidness and cost effectiveness, but also its potential application as a rapid, potentially automated, online monitoring technique for the routine analysis of FA composition in industrial processes when used together with the multivariate data analysis modelling.