Genome-wide analysis of KAP1, the 7SK snRNP complex, and RNA polymerase II

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• 作者：Ryan P. McNamara^a ; ¹ ; ² ; Carlos Guzman^a ; ² ; Jonathan E. Reeder^b ; Ivá ; n D'Orso^a ; ^{ivan.dorso@utsouthwestern.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：P-TEFb ; positive transcription elongation factor b ; Pol II ; RNA polymerase II ; TSS ; transcription start site ; Bp ; base pairs ; KAP1 ; Kruppel-associated protein ; Cdk9 ; cyclin-dependent kinase 9 ; CycT1 ; cyclin T1 ; MePCE ; methyl phosphate capping enzyme ; ChIP ; chromatin immunoprecipitation ; snRNP ; small nuclear ribonucleoprotein ; KEC ; KAP1-7SK snRNP early elongation complex ; Larp7 ; La-related protein 7 ; Hexim1 ; hexamethylene bis-acetamide inducible protein 1 ; qPCR ; quantitative PCR ; ChIP-seq ; chroma
• 刊名：Genomics Data
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：7
• 期：Complete
• 页码：250-255
• 全文大小：926 K

文摘

The transition of RNA polymerase II (Pol II) from transcription initiation into productive elongation in eukaryotic cells is regulated by the P-TEFb kinase, which phosphorylates the C-terminal domain of paused Pol II at promoter-proximal regions. Our recent study found that P-TEFb (in an inhibited state bound to the 7SK snRNP complex) interacts with the KAP1/TRIM28 transcriptional regulator, and that KAP1 and the 7SK snRNP co-occupy most gene promoters containing paused Pol II. Here we provide a detailed experimental description and analysis of the ChIP-seq datasets that have been deposited into Gene Expression Omnibus (GEO): GS72622, so that independent groups can replicate and expand upon these findings. We propose these datasets would provide valuable information for researchers studying mechanisms of transcriptional regulation including Pol II pausing and pause release.