Physical and genetic map of the Bacteroides fragilis YCH46 chromosome

详细信息    查看全文

• 作者：Kuwahara ; Tomomi ; Sarker ; Mahfuzur Rahman ; Ugai ; Hideyo ; Akimoto ; Shigeru ; Shaheduzzaman ; Syed Mohammed ; et. al.
• 关键词：Physical map ; Pulsed-field gel electrophoresis ; Bacteroides fragilis
• 刊名：FEMS Microbiology Letters
• 出版年：2002
• 出版时间：February 5, 2002
• 年：2002
• 卷：207
• 期：2
• 页码：193-197
• 全文大小：222 K

文摘

The chromosome of Bacteroides fragilis strain YCH46 was shown to be a single circular DNA molecule of about 5.3 Mb having 16 NotI, seven AscI, and six I-CeuI sites. A physical map of the chromosome was constructed by four independent experimental approaches: linking clone analysis, cross-Southern hybridization, partial restriction digestion, and two-dimensional pulsed-field gel electrophoresis. Six rRNA operons and 10 known genes were localized on the physical map.