K-562 cells lack MHC class II expression due to an alternatively spliced CIITA transcript with a truncated coding region

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• 作者：Day ; Noel E. ; Ugai ; Hideyo ; Yokoyama ; Kazunari K. ; Ichiki ; Albert T.
• 关键词：K-562 ; CIITA ; MHC class II ; CIITA promoter-IV ; IFN-γ ; -inducible
• 刊名：Leukemia Research
• 出版年：2003
• 出版时间：November, 2003
• 年：2003
• 卷：27
• 期：11
• 页码：1027-1038
• 全文大小：410 K

文摘

The focus of this study was to determine the functional capacity of class II transactivator (CIITA), a regulatory factor of major histocompatibility complex (MHC) class II genes, in K-562 cells. We show that CIITA mRNA is present in K-562 cells and the interferon-γ (IFN-γ)-inducible CIITA promoter-IV exhibits low levels of basal activity, which is greatly enhanced upon treatment with IFN-γ. Further study revealed that the CIITA cDNA contains an insertion of genomic sequence, which introduces a stop codon. The truncated coding region of the CIITA transcript in K-562 cells provides a possible explanation for the absence of MHC class II molecules.