Generation of cloned calves and transgenic chimeric embryos from bovine embryonic stem-like cells

详细信息    查看全文

• 作者：Saito ; Shigeo ; Sawai ; Ken ; Ugai ; Hideyo ; Moriyasu ; Satoru ; Minamihashi ; Akira ; Yamamoto ; Yusuke ; et. al.
• 关键词：Bovine ; ES-like cells ; In vitro differentiation ; Cloning ; Nuclear transplantation
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2003
• 出版时间：September 12, 2003
• 年：2003
• 卷：309
• 期：1
• 页码：104-113
• 全文大小：206 K

文摘

Bovine embryonic stem-like cells (ES-like cells) appear to maintain a normal diploid karyotype indefinitely during culture in vitro and to express marker proteins that are characteristic of ES cells from mice, namely, alkaline phosphatase (AP), stage-specific embryonic antigen-1 (SSEA-1), STAT-3, and Oct 4. After proliferation of undifferentiated ES-like cells in vitro, some bovine ES-like cells differentiated to neural precursor cells, which were cultured in the presence of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), and platelet-derived growth factor (PDGF). In addition, calves were successfully cloned using ES-like cells and the frequency of term pregnancies for blastocysts derived from ES-like cells was higher than those of early pregnancies and maintained pregnancies after nuclear transplantation (NT) with bovine somatic cells. Successful cloning from bovine ES-like cells should allow the introduction into cattle of specific genetic characteristics of biomedical and/or agricultural importance.