In vitro uptake of [1-¹⁴C]Octanoate in brain slices of rats: basic studies for assessing [1-¹¹C]Octanoate as a PET tracer of glial functions

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• 作者：Kuge ; Yuji ; Hikosaka ; Kenji ; Seki ; Koh-ichi ; Ohkura ; Kazue ; Nishijima ; Ken-ichi ; Tsukamoto ; Eriko ; et. al.
• 关键词：[1-¹¹C]octanoate ; Glial function ; Brain slice ; Rat ; Positron emission tomography
• 刊名：Nuclear Medicine and Biology
• 出版年：2002
• 出版时间：April, 2002
• 年：2002
• 卷：29
• 期：3
• 页码：303-306
• 全文大小：121 K

文摘

To clarify the contribution of glial cells to octanoate uptake into the brain, we determined the effects of fluoroacetate, a selective inhibitor of glial metabolism, on in vitro brain uptake of [1-¹⁴C]octanoate, using rat brain slices. The [1-¹⁴C]octanoate uptake significantly decreased, depending on the concentration of fluoroacetate (p = 0.001). The [1-¹⁴C]octanoate uptakes at 5 mM (0.23 ± 0.05 % uptake/mg slice) and 25 mM fluoroacetate (0.12 ± 0.01 % uptake/mg slice) were significantly lower than that at control (0.29 ± 0.02 % uptake/mg slice, p < 0.05 and p < 0.001, respectively). The results demonstrate the contribution of glial cells to octanoate uptake into the brain. The potential of [1-¹¹C]octanoate as a PET tracer for studying glial functions is suggested.