Hydrogen peroxide-induced Ca²⁺ responses in CNS pericytes

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• 作者：Masahiro Kamouchi ; Takanari Kitazono ; Tetsuro Ago ; Masanori Wakisaka ; Junya Kuroda ; Kuniyuki Nakamura ; Noriko Hagiwara ; Hiroaki Ooboshi ; Setsuro Ibayashi ; Mitsuo Iida
• 关键词：Cerebrovascular circulation ; Reactive oxygen species ; Calcium signaling ; Microcirculation
• 刊名：Neuroscience Letters
• 出版年：2007
• 出版时间：6 April 2007
• 年：2007
• 卷：416
• 期：1
• 页码：12-16
• 全文大小：384 K

文摘

Objective: The aims of the present study were to elucidate the interaction of reactive oxygen species (ROS) and Ca²⁺ response in central nervous system (CNS) pericytes. Methods: The intracellular Ca²⁺ concentration was measured using fluorescent Ca²⁺ indicator, fura-2, in cultured CNS pericytes. Results: Hydrogen peroxide evoked a dose-dependent increase in cytosolic Ca²⁺, which was completely inhibited by catalase. Removal of external Ca²⁺ or addition of nicardipine (1 μM) during application of hydrogen peroxide did not affect Ca²⁺ response. Incubation of the cells in Ca²⁺ free solution did not abolish but slightly reduced Ca²⁺ response by hydrogen peroxide. Ca²⁺ response to hydrogen peroxide was not altered by the depletion of intracellular Ca²⁺ by thapsigargin (1 μM). Pretreatment of the cells with tyrosine kinase inhibitor genistein (100 μM) or tyrphostin A47 (30 μM) significantly reduced Ca²⁺ increase by hydrogen peroxide. Conclusions: These results indicate that hydrogen peroxide evokes Ca²⁺ increase predominantly by release from intracellular Ca²⁺ store, which may be regulated by tyrosine kinases.