In vitro grown thickened taproots, a new type of soil transplanting source in Panax ginseng

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• 作者：Jong Youn Kim¹ ; Dong Hwi Kim² ; Young Chang Kim² ; Kee Hong Kim² ; Jung Yeon Han¹ ; Yong Eui Choi¹ ; ^{yechoi@kangwon.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：in vitro taproot thickening ; micropropagation ; Panax ginseng ; soil transfer
• 刊名：Journal of Ginseng Research
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：40
• 期：4
• 页码：409-414
• 全文大小：1436 K

文摘

The low survival rate of in vitro regenerated Panax ginseng plantlets after transfer to soil is the main obstacle for their successful micropropagation and molecular breeding. In most cases, young plantlets converted from somatic embryos are transferred to soil.

Methods

In vitro thickened taproots, which were produced after prolonged culture of ginseng plantlets, were transferred to soil.

Results

Taproot thickening of plantlets occurred near hypocotyl and primary roots. Elevated concentration of sucrose in the medium stimulated the root thickening of plantlets. Senescence of shoots occurred following the prolonged culture of plantlets. Once the leaves of plantlets senesced, the buds on taproots developed a dormant tendency. Gibberellic acid treatment was required for dormancy breaking of the buds. Analysis of endogenous abscisic acid revealed that the content of abscisic acid in taproots with senescent shoots was comparatively higher than that of taproots with green shoots. Thickened taproots were transferred to soil, followed by exposure to gibberellic acid or a cold temperature of 2°C for 4 mo. Cold treatment of roots at 2°C for 4 mo resulted in bud sprouting in 84% of roots. Spraying of 100 mg/L gibberellic acid also induced the bud sprouting in 81% roots.

Conclusion

Soil transfer of dormant taproots of P. ginseng has advantages since they do not require an acclimatization procedure, humidity control of plants, and photoautotrophic growth, and a high soil survival rate was attained.