A surface plasmon resonance–based system to genotype human papillomavirus

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• 作者：Shuyu Wang ; Huawei Yang ; Hongtao Zhang ; Fuhui Yang ; Mingshu Zhou ; Chanwei Jia ; Yonglian Lan ; Yanmin Ma ; Liying Zhou ; Suer Tian ; Sheng Wang ; Hongen Zhang ; Zhong Chen
• 刊名：Cancer Genetics and Cytogenetics
• 出版年：2010
• 出版时间：15 July 2010
• 年：2010
• 卷：200
• 期：2
• 页码：100-105
• 全文大小：284 K

文摘

We report a novel system (W2600) that is based on the technology of surface plasmon resonance (SPR) to genotype human papillomavirus (HPV). The system permitted detection of 24 known HPV genotypes, including 16 high-risk types (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 81) and 8 low-risk types (HPV 6, 11, 40, 42, 43, 44, 54, 70). Analytical performance of W2600 for HPV genotyping was evaluated by HPV DNA derived from the liquid cervical cytology specimens of 560 patients with atypical squamous cells of undetermined significance or above. In comparison with clonal sequence analysis, 358 of 560 (64 % ) and 355 of 560 (63 % ) cases were found to be positive within the 24 HPV genotypes by W2600 and sequence analysis, respectively. Concordance between these two methods was at 555 of 560 (99 % ) (kappa = 0.98, P < 0.001); only 5 of the 560 (1 % ) cases had discordant results. No cross-hybridizations were observed with the W2600 system, and the spectrum of HPV genotypes identified by W2600 included all the 16 high-risk genotypes. These data demonstrate that the SPR-based W2600 system is highly sensitive and specific in HPV genotyping and can provide an effective approach for such application in a clinical setting.