Separation of enzymatic functions and variation of spin state of rice allene oxide synthase-1 by mutation of Phe-92 and Pro-430
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- 作者：Sereyvath Yoeuna ; Andrey Sukhanovb ; Oksoo Hana ; oshan@chonnam.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：AOS ; allene oxide synthase ; AOC ; allene oxide cyclase ; LOX ; lipoxygenase ; HPL ; hydroperoxide lyase ; HPOD ; hydroperoxydienoic acid ; HPOT ; hydroperoxytrienoic acid ; EOD ; 12 ; 13-epoxy-9 ; 11-octadecadienoic acid ; EOT ; 12 ; 13-epoxy-9 ; 11 ; 15-octadecatrienoic acid ; LA ; linoleic acid ; LnA ; linolenic acid ; (9Z)-α-ketol ; (9Z)-13-hydroxy-12-oxo-9-ocadecenoic acid ; (12Z)-α-ketol ; (12Z)-9-hydroxy-10-oxo-12-octadecenoic acid) ; (10E)-γ-ketol ; (10E)-9-hydroxy-12-oxo-9-octadecenoic acid ; (10E)-γ-ketol ; (11E)-13-hydr
- 刊名：Bioorganic Chemistry
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：68
- 期：Complete
- 页码：9-14
- 全文大小：1579 K
文摘
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AOS and HPL branch prefers 13-HPOT and 13-HPOD for substrates, respectively.
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High and low spin states are in equilibrium in wild type, F92L and P430A mutants.
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Inhibitory effect of imidazole is more effective for AOS mechanism than HPL mechanism.
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Type II ligands may regulate flux through AOS or HPL branch in oxylipin pathway.