Development and validation of a quantitative competitive ELISA for potency testing of equine anti rabies sera with other potential use
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- 作者：Jehanara Korimbocusa ; Nicolas Dehayb ; Noë ; l Tordoc ; Franç ; ois Canoa ; Sylvie Morgeauxa ; sylvie.morgeaux@ansm.sante.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Competitive ELISA ; Rabies immunoglobulins (ERIGs ; HRIGs) ; Quality control ; Validation ; 3Rs ; Batch release
- 刊名：Vaccine
- 出版年：2016
- 出版时间：14 June 2016
- 年：2016
- 卷：34
- 期：28
- 页码：3310-3316
- 全文大小：1253 K
文摘
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An in vitro c-ELISA method was tested in view of replacing the in vivo MNT assay.
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This ELISA is able to detect equine immunoglobulin against rabies with neutralising activity.
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As the MNT test this c-ELISA is able to discriminate non-compliant vaccine batches.
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The c-ELISA is a potential tool for detection of human immunoglobulin against rabies.