Spectral and morphological classification of different chronic and acute Taiwanese gallstones via FTIR, SEM and ESEM-EDX microanalyses

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• 作者：Ching-Li Cheng^a ; Hsiao-Huang Chang^b ; ^c ; ¹ ; ^{shchang@vghtpe.gov.tw" class="auth_mail" title="E-mail the corresponding author} ; Tien-Hua Chen^b ; Pei-Jiun Tsai^b ; Yu-Ting Huang^d ; Pei-Jung Huang^b ; Shan-Yang Lin^d ; ¹ ; ^{sylin@mail.ypu.edu.tw" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Acute gallbladder sludge ; Chronic gallstone ; EDX ; ESEM ; FTIR ; SEM
• 刊名：Digestive and Liver Disease
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：48
• 期：5
• 页码：519-527
• 全文大小：2284 K

文摘

Gallstone disease is one of the leading upper gastrointestinal surgical problems in different countries.

Aims

To analyze the chronic gallstones and acute gallbladder sludge retrieved from 36 Taiwanese patients.

Methods

FTIR microspectroscopy was used to classify the types of gallstones, and an ESEM-EDX microanalysis was first applied to determine the microstructural features and elemental compositions of the various gallstones. Bacteria presented on the surface of gallstones were also detected by SEM.

Results

Four types of gallstones were obtained from these 36 Taiwanese patients: calcium bilirubinate (CaBR) stones (30.6%), cholesterol stones (19.4%), mixed stones including 6 subtypes (47.2%), and acute gallbladder sludge (2.8%) made of CaBR and protein/insoluble biomaterials. Bacteria imprints and bacterial discharges or bacterial biofilms were also found on the surface of gallstones and acute sludge under a SEM observation. ESEM-EDX results revealed that calcium was found to be the main constituent of all of the types of stones except cholesterol stones, and aluminum was also presented in most of the stones and sludge samples. Chloride was only detected in the acute gallbladder sludge.

Conclusion

FTIR spectra, morphological features, and elemental compositions of the acute gallbladder sludge were different from those of the chronic gallstones.