A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase
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- 作者：Gang Gao1 ; An Wang1 ; Bo-Liang Gong ; Qing-Qing Li ; Yu-Huan Liu ; Zhu-Mei He ; lsshezm@mail.sysu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Gang Li ; lsslig@mail.sysu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Coptotermes formosanus ; Metagenomic library ; Phosphotransferase system ; Glucosidase ; Glucose tolerance
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：84
- 期：Complete
- 页码：24-31
- 全文大小：1644 K
文摘
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A novel PTS related gene cluster with overlapped gene was found from metagenomic library of termite hindgut.
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There was an interaction between the PTS components Glu1392 and Glu1923, and the co-expression of these two components was responsible for the utilization of the glucoside substrates in E. coli.
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The glucosidase Glu1392 had a very high tolerance to glucose with a Ki value of 2.25 M.