Identification of the linear epitope for Fc-binding on the bovine IgG2 Fc receptor (boFcγ2R) using synthetic peptides

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• 作者：Gaiping Zhang ; Junqing Guo ; Jiyong Zhou ; Xuannian Wang ; Qingmei Li ; Yanyan Yang ; Huigang Shen ; Dong Zhao ; Hua Zhang ; Jun Xi et al.
• 关键词：Fc-binding epitope ; Bovine IgG2 receptor ; Bovine IgG2 ; Synthetic peptides
• 刊名：FEBS Letters
• 出版年：2006
• 出版时间：20 February 2006
• 年：2006
• 卷：580
• 期：5
• 页码：1383-1390
• 全文大小：286 K

文摘

To identify the linear epitope for Fc-binding on the bovine IgG2 Fc receptor (boFcγ2R), peptides derived from the membrane-distal extracellular domain (EC1) of boFcγ2R corresponding to the homologous region of human FcαRI were synthesized. Binding of bovine IgG2 to the different peptides was tested by Dot-blot assay, and the peptide showing maximal binding was further modified by truncation and mutation. The minimum effective peptide ⁸²FIGV⁸⁵ located in the putative F–G loop of the EC1 domain was found to bind bovine IgG2 specifically and inhibit the binding of bovine IgG2 to the receptor. The Phe⁸², Ile⁸³ and Val⁸⁵ residues within the linear epitope were shown to be critical for IgG2-binding. Such functional epitope peptide should be very useful for understanding the IgG-Fcγ interaction and development of FcR-targeting drugs.