Adenosine-triphosphate-sensitive K⁺channel (Kir6.1): A novel phosphospecific interaction partner of connexin 43 (Cx43)

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• 作者：Ajaz Ahmad Waza ; Khurshid Andrabi ; Mahboob Ul Hussain
• 关键词：Connexin 43 ; Kir6.1 ; Ischemia preconditioning ; Phosphorylation
• 刊名：Experimental Cell Research
• 出版年：2012
• 出版时间：10 December, 2012
• 年：2012
• 卷：318
• 期：20
• 页码：2559-2566
• 全文大小：581 K

文摘

Connexin 43 (Cx43) is a phosphoprotein expressed in a wide variety of cells. Cx43 and adenosine-triphosphate-sensitive K⁺channels [K⁺(ATP)] are part of same signaling pathway that regulates cell survival during stress and ischemia preconditioning. Molecular mechanism for their coordinated role in ischemia/hypoxia preconditioning is not well known. Using pull down, co-immunoprecipitation assays and co-localization studies we provide evidence, for the first time that Kir6.1, a K⁺(ATP) channel protein component, can interact with Cx43. Further we show that the interaction was phospho-specific such that Cx43 phosphorylated at serine 262 (S262) interacted with Kir6.1 in preference to the unphosphorylated form of Cx43. Introduction of phospho-deficient mutation at serine 262 (S262A) in Cx43 completely abolished the interaction. Our data provide an interesting lead about a possible partnership between Cx43 and Kir6.1, which will help in better understanding their role in ischemia/hypoxia preconditioning.