Investigation of kinetics and thermodynamics of DNA hybridization by means of 2-D fluorescence spectroscopy and soft/hard modeling techniques
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- 作者：Sara Ebrahimi ; Mohsen Kompany-Zareh ; kmpz@dr.com" class="auth_mail" title="E-mail the corresponding author ; kompanym@iasbs.ac.ir" class="auth_mail" title="E-mail the corresponding author
- 关键词：DNA hybridization ; Kinetics ; Fluorescence ; Hard modeling ; Multiway analysis
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：4 February 2016
- 年：2016
- 卷：906
- 期：Complete
- 页码：58-71
- 全文大小：4140 K
文摘
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Hard restricted trilinear decomposition (HrTD) was introduced for model-based analysis of three-way rank deficient data.
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DNA hybridization was investigated by two-dimensional fluorescence spectroscopy and soft/hard multi-way techniques.
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Restricted Tucker3 analysis enabled accurate estimation of pure FRET profiles in the hybridized form.
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HrTD was successfully employed to estimate kinetic and equilibrium parameters of DNA hybridization system.
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The performance of the proposed methods in response to different physical stimuli was successfully evaluated.