ω-Hydroxylation of docosahexaenoic acid or arachidonic acid in human colonic well differentiated adenocarcinoma homogenate
详细信息 查看全文
- 作者：Shimizu ; Shinsuke ; Yamane ; Mototeru ; Abe ; Akihisa ; Nakajima ; Masato ; Sugiura ; Hirokazu ; Miyaoka ; Masaaki ; et. al.
- 关键词：ω ; -Hydroxydocosahexaenoic acid ; ω ; -Hydroxyeicosatetraenoic acid ; ω ; -Oxidation ; Human colonic well differentiated adenocarcinoma ; Liquid chromatography-thermospray-mass spectrometry
- 刊名：Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
- 出版年：1995
- 出版时间：June 6, 1995
- 年：1995
- 卷：1256
- 期：3
- 页码：293-296
- 全文大小：337 K
文摘
Human colonic well differentiated adenocarcinoma homogenate was incubated with NADPH and docosahexaenoic acid (22:6(n - 3)) or arachidonic acid (20:4(n - 6)) as a substrate. On a selected ion monitoring chromatogram obtained with reversed phase-high-performance liquid chromatography thermospray mass spectrometry, ω-hydroxydocosahexaenoic acid (ω-HDHE) or ω-hydroxyeicosatetraenoic acid (ω-HETE) from an incubation mixture of the homogenate was detected in significant amount, compared to that from a colonic region remote from the carcinoma. In contrast, epoxydocosapentaenoic acids and the dihydroxy derivatives from 22:6(n - 3) or epoxyeicosatrienoic acids and the dihydroxy derivatives from 20:4(n - 6) were detected in low amounts, compared to that from a colonic region remote from the carcinoma. The results suggest that highly active NADPH-dependent ω-oxidations of polyunsaturated fatty acids occur in colonic adenocarcinoma homogenate.