Chitosan-g-hematin: Enzyme-mimicking polymeric catalyst for adhesive hydrogels

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• 作者：Ji Hyun Ryu ; Yuhan Lee ; Min Jae Do ; Sung Duk Jo ; Jee Seon Kim ; Byung-Soo Kim ; Gun-Il Im ; Tae Gwan Park ; Haeshin Lee
• 关键词：Chitosan ; Hematin ; Horseradish peroxidase ; Catechol ; Hydrogel
• 刊名：Acta Biomaterialia
• 出版年：January, 2014
• 年：2014
• 卷：10
• 期：1
• 页码：224-233
• 全文大小：1756 K

文摘

Phenol derivative-containing adhesive hydrogels has been widely recognized as having potential for biomedical applications, but their conventional production methods, utilizing a moderate/strong base, alkaline buffers, the addition of oxidizing agents or the use of enzymes, require alternative approaches to improve their biocompatibility. In this study, we report a polymeric, enzyme-mimetic biocatalyst, hematin-grafted chitosan (chitosan-g-hem), which results in effective gelation without the use of alkaline buffers or enzymes. Furthermore, gelation occurs under mild physiological conditions. Chitosan-g-hem biocatalyst (0.01%, w/v) has excellent catalytic properties, forming chitosan-catechol hydrogels rapidly (within 5 min). In vivo adhesive force measurement demonstrated that the hydrogel formed by the chitosan-g-hem activity showed an increase in adhesion force (33.6 卤 5.9 kPa) compared with the same hydrogel formed by pH-induced catechol oxidation (20.6 卤 5.5 kPa) in mouse subcutaneous tissue. Using the chitosan-g-hem biocatalyst, other catechol-functionalized polymers (hyaluronic acid-catechol and poly(vinyl alcohol)-catechol) also formed hydrogels, indicating that chitosan-g-hem can be used as a general polymeric catalyst for preparing catechol-containing hydrogels.