Anti-idiotypic VHH phage display-mediated immuno-PCR for ultrasensitive determination of mycotoxin zearalenone in cereals
详细信息 查看全文
- 作者：Xianxian Wanga ; b ; c ; Qinghua Hec ; heqinghua@ncu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yang Xub ; c ; Xing Liub ; c ; Mei Shub ; c ; Zhui Tub ; Yanping Lic ; Wei Wangb ; c ; Dongmei Caob ; c
- 关键词：Zearalenone ; Anti-idiotypic antibody ; Immuno-polymerase chain reaction
- 刊名：Talanta
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：147
- 期：Complete
- 页码：410-415
- 全文大小：1124 K
文摘
- •
Anti-idiotypic VHH phage can be used as non-toxin substitute for ZEN detection.
- •
We developed an anti-idiotypic VHH based phage-ELISA for detection of ZEN in cereals.
- •
We developed an ultrasensitive and specific PD-IPCR for ZEN determination in cereals.
- •
PD-IPCR improved the LOD by 12-fold than phage ELISA results.
- •
Compared with phage ELISA results, PD-IPCR expanded the linear range considerably.