Anti-idiotypic VHH phage can be used as non-toxin substitute for ZEN detection.
We developed an anti-idiotypic VHH based phage-ELISA for detection of ZEN in cereals.
We developed an ultrasensitive and specific PD-IPCR for ZEN determination in cereals.
PD-IPCR improved the LOD by 12-fold than phage ELISA results.
Compared with phage ELISA results, PD-IPCR expanded the linear range considerably.