Proteomic and bioinformatic analysis of a nuclear intrinsically disordered proteome
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- 作者：Bozena Skupien-Rabiana ; b ; Urszula Jankowskaa ; b ; Bianka Swiderskaa ; Sylwia Lukasiewicza ; Damian Ryszawyc ; Marta Dziedzicka-Wasylewskaa ; Sylwia Kedracka-Kroka ; b ; sylwia.kedracka-krok@uj.edu.pl" class="auth_mail" title="E-mail the corresponding author
- 关键词：IDPs ; intrinsically disordered proteins ; IDP-ome ; intrinsically disordered proteome ; 2-DE ; two-dimensional gel electrophoresis ; MudPIT ; multidimensional protein identification technology ; LC&ndash ; MS/MS ; liquid chromatography coupled with tandem mass spectrometry ; nanoHPLC ; nano high-performance liquid chromatography ; WCL ; whole cell lysate ; WCL_IDPs ; whole cell lysate enriched in IDPs ; N ; nuclear lysate ; N_IDPs ; nuclear lysate enriched in IDPs ; DRs ; disordered residues ; SPL ; scheduled precursor
- 刊名：Journal of Proteomics
- 出版年：2016
- 出版时间：1 January 2016
- 年：2016
- 卷：130
- 期：Complete
- 页码：76-84
- 全文大小：840 K
文摘
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The first large-scale experimental analysis of a nuclear IDP subproteome is presented.
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Experimental evidence that IDPs are overrepresented in cell nuclei is provided.
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Nuclear IDPs are shown to be enriched in proteins involved in transcription regulation.
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A method for differential proteomic studies of the nuclear IDP-ome is proposed.