Combining two serological assays optimises sensitivity and specificity for the identification of Streptococcus equi subsp. equi exposure

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• 作者：Carl Robinson ; Karen F. Steward ; Nicola Potts ; Colin Barker ; Toni-ann Hammond ; Karen Pierce ; Eggert Gunnarsson ; Vilhj¨¢lmur Svansson ; Josh Slater ; J. Richard Newton ; Andrew S. Waller
• 关键词：Horses ; Strangles ; Streptococcus equi ; iELISA
• 刊名：The Veterinary Journal
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：197
• 期：2
• 页码：188-191
• 全文大小：396 K

文摘

The detection of anti-Streptococcus equi antibodies in the blood serum of horses can assist with the identification of apparently healthy persistently infected carriers and the prevention of strangles outbreaks. The aim of the current study was to use genome sequencing data to develop an indirect enzyme linked immunosorbent assay (iELISA) that targets two S. equi-specific protein fragments. The sensitivity and specificity of the antigen A and antigen C iELISAs were compared to an SeM-based iELISA marketed by IDvet - diagnostic V¨¦t¨¦rinaire (IDvet). Individually, each assay compromised specificity in order to achieve sufficient sensitivity (SeM iELISA had a sensitivity of 89.9 % , but a specificity of only 77.0 % ) or sensitivity to achieve high specificity. However, combining the results of the antigen A and antigen C iELISAs permitted optimisation of both sensitivity (93.3 % ) and specificity (99.3 % ), providing a robust assay for the identification of horses exposed to S. equi.