The influence of hydro-ethanolic extract of Portulaca oleracea L. on Th₁/Th₂ balance in isolated human lymphocytes

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• 作者：Vahid Reza Askari^a ; Seyed Abdolrahim Rezaee^b ; Khalil Abnous^d ; Mehrdad Iranshahi^e ; Mohammad Hossein Boskabady^c ; ^{boskabadymh@mums.ac.ir} ; ^{mhboskabady@hotmail.com}
• 关键词：Portulaca oleracea L. ; Th1/Th2 (IFN-γ/IL-4) balance ; Treg/Th2 (IL-10/IL-4) balance ; Nitric Oxide ; IL-4 ; IFN-γ ; IL-10
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：24 December 2016
• 年：2016
• 卷：194
• 期：Complete
• 页码：1112-1121
• 全文大小：1002 K
• 卷排序：194

文摘

The anti-inflammatory and anti-oxidants activity of Portulaca oleracea L. (P. oleracea) were mentioned in traditional texts. In previous studies, different anti-inflammatory and anti-oxidant effects of P. oleracea were demonstrated. However, the mechanism of action and immunomodulatory property of this plant are greatly unknown. In the present study, the effect of the extract of this plant on IL-4, IL10, IFN-γ and T helper (h)1/Th2 balance in non-stimulated and stimulated human lymphocytes was examined.Materials and methodsThe effect of three concentrations (160, 40 and 10 µg/ml) of P. oleracea or dexamethasone were evaluated on percentage of cell proliferation and nitric oxide (NO) production as well as secretion of cytokines (IL-4, IL10 and IFN-γ) in PHA-stimulated and non-stimulated lymphocytes, and compared to control and dexamethasone as positive control (n=15 for each group).ResultsIn stimulated cells, dexamethasone significantly inhibited the percentage of cell proliferation, NO production, and secretion of cytokines in comparison to control group (P<0.001 for all cases). The percentage of cell proliferation, NO production, and secretion of cytokines were significantly decreased while Th1/Th2 (IFN-γ/IL-4) and Treg/Th2 (IL-10/IL-4) balances significantly enhanced in treated groups with all three concentrations of extract compared to control group (P<0.001 for all cases). The effect of all concentrations of the extract on cell proliferation, NO production and secretion of cytokines as well as Treg/Th2 balance were significantly lower than dexamethasone (P<0.001 for all cases), but Th1/Th2 ratio obtained in the presence of only low extract concentration was lower than dexamethasone (P<0.01).ConclusionDifferent concentrations of extract promoted Th1/Th2 and Treg/Th2 balances which may suggest the therapeutic value of the plant in inflammatory disease associated with decreased Th1/Th2 balance such as asthma or cancers.