In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO₂: HA formation, cellular affinity and organ culture

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• 作者：Sang-Hyun An ; Takuya Matsumoto ; Jun-Ichi Sasaki ; Hiroyuki Miyajima ; Ramaswamy Narayanan ; Satoshi Imazato ; Kyo-Han Kim
• 关键词：Surface modification ; Anodization ; Nano-surface ; Micro-surface ; In vitro study
• 刊名：Materials Science and Engineering: C
• 出版年：2012
• 出版时间：1 December, 2012
• 年：2012
• 卷：32
• 期：8
• 页码：2516-2522
• 全文大小：1332 K

文摘

Anodization is an easily viable technique useful for producing TiO₂ coatings on titanium substrates. Nano-crystalline anodic TiO₂ structure was produced on titanium at 20 V using 1 M Na₂SO₄ and 0.5 % NaF and consolidated by a further heat-treatment. Micro-crystalline anodic TiO₂ was produced on titanium by applying a galvanostatic current density of 70 A/m² in water medium. To assess the usefulness of these nano- and micro-oxides for bone implant stability, physical properties and bone in vitro bioactivity including HA formation, cellular affinity and mouse-tissue morphogenesis, were evaluated. Bioactivity of the different anodic surfaces was evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the nano-oxide surface within 7 days, whereas HA was detected only after 14 days on the micro-oxide surface. In vitro cell culture tests done using mouse osteoblasts indicated that the nano-oxides showed statistically significant cell activity than the micro-oxides and the machined titanium. Branching morphogenesis test done for 72 h on these surfaces showed more branching on the micro- and nano-oxides as compared with titanium surface.